Journal: Cell Reports
Article Title: K13-Mediated Reduced Susceptibility to Artemisinin in Plasmodium falciparum Is Overlaid on a Trait of Enhanced DNA Damage Repair
doi: 10.1016/j.celrep.2020.107996
Figure Lengend Snippet: Artesunate-Induced DNA Damage in P. falciparum Lab Strains (A) Schematic illustration of artesunate treatment based on Streptolysin O (SLO) enrichment (ring stage) or Percoll gradient centrifugation (schizont stage). (B) Artesunate-induced DNA damage in P. falciparum 3D7, Dd2, and Dd2 I543T . Ring-stage (upper panel) and schizont-stage (lower panel) P. falciparum parasites were treated with different concentrations of artesunate for 1 h and subjected to analysis on alkaline CometChip. On average, ~302 comets were analyzed per macrowell. Each experiment was done in technical triplicates, and data here were collected and are shown in biological triplicates or quadruplicates. ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001, one-way ANOVA. Dunnett’s multiple comparison tests were performed between vehicle control (0 μM) and respective concentrations. All data are indicated as means ± SEM.
Article Snippet: CometChip raw data and analyzed results, Part five , This paper; Mendeley data , https://doi.org/10.17632/8gsnzy5ndj.1.
Techniques: Gradient Centrifugation, Comparison, Control